In 1869, Justin Eagle lives on his ranch called "The Eagle's Nest" near the town of Button Willow, California. In addition to being a rancher, Juston is a trouble-shooter for the U. S. Government which calls for him to act as an undercover operative and thwart the forces of evil in the rapidly-growing West. He is sent to San Franciso to find missing U. S. Senaator Freeman, who has disappeared while fighting the efforts of Montgomery Blaine, a villain who has been, with the aid of his henchman, "The Whip," forcing settlers to sell their land to him, not knowing that the land is in the path of a proposed railroad, from Utah, that will link the western United States to the East. Senator Freeman is the leader of an effort to veer the railroad southward to bypass Blaine's land and, for his efforts, is kidnapped by Bliane's henchmen and shanghaied from the San Francisco waterfront. Justin Eagle's job is to find and return him safely.

Sylvester Cat and his son, Junior, live in a dump, and Junior decides to find them a home. He does, but the fat lady who lives there only wants to adopt Junior and separates the kitten from his father. So, Sylvester makes a number of attempts to gain access to her house.

A man waits on death row while his son and friend try to prove that he did not kill a grocer with an ax.

The warping lens used to photograph 10th Avenue seems to puzzle the filmmakers.

A showman invites patrons to step up to his Kinetoscope-style magic box and glimpse the answers to their most burning questions.

Melchiors bride Keterlyn happens to witness an attack on a young man. The victim, who has lost his memory, does not know who he is and how he ended up in a Tallinn. A ring found at the scene leads Melchior to the trail of the nocturnal brotherhood, questions are also raised by the troupe of freaks who performed on fair day. Ominous signs suggest that not only is the stranger recovering from a murder attempt at risk, but Melchior and Keterlyn as well. At the same time, an inquisitor known as a ruthless witch hunter arrives in town. Melchior's ability to "speak to the dead" and to notice invisible traces of murder have raised the suspicion that he may be a dangerous heretic. And the place of the enemy of the church is at the stake